By Rogerio Jelmayer And Jeffrey T. Lewis 

SÃO PAULO--A widening investigation into alleged corruption at Brazil's state-controlled oil company Petróleo Brasileiro SA took a step closer to President Dilma Rousseff Wednesday morning when police arrested her political party's treasurer, João Vaccari Neto.

Mr. Vaccari has been charged with receiving "irregular donations" for the Workers' Party, or PT, from some of the oil company's suppliers. Police allege the suppliers inflated contracts with Petrobras, funneling some of the proceeds to politicians and election campaigns.

Pedro Barusco, a former manager of Petrobras's engineering and services department who is cooperating with prosecutors, told a congressional hearing in March that he amassed nearly $100 million in bribes as part of the alleged scheme and the PT may have received twice as much. Mr. Barusco told lawmakers he used to meet with Mr. Vaccari in high-end hotels or restaurants to discuss how to share bribes, and that some of the money was passed on to Ms. Rousseff's 2010 election campaign.

Mr. Vaccari testified last week before a congressional committee investigating the allegations and denied accepting any illegal donations. Ms. Rousseff has denied any involvement in or knowledge of the alleged corruption. Brazil's Attorney General Rodrigo Janot said in March that there are no facts to motivate an investigation of the president.

The president's office referred requests for comment on Mr. Vaccari's arrest to the PT, and the PT had no immediate comment. Attempts to contact Mr. Vaccari or his lawyer were unsuccessful.

The country's Supreme Court in March approved investigations into dozens of sitting congressmen, including members of Ms. Rousseff's Workers Party. Last week, police said they found evidence of similar scams at Brazil's health ministry and at state-owned bank Caixa Econômica Federal.

Both the health ministry and Caixa said they would investigate the allegations and are cooperating with police and prosecutors. Caixa said Tuesday that according to reports from the Federal Police and the prosecutors' office, there are "no indications of irregular practices" by the bank or its employees.

The arrest of Mr. Vaccari is likely to spur renewed calls for the president to be impeached. Thousands of Brazilians have taken to the streets on two recent occasions to protest corruption in the government. A survey released last weekend by the Datafolha polling company said 63% of those asked now support impeachment and put the president's approval rating at 13%.

Senator Aecio Neves, who lost to Ms. Rousseff in last year's presidential election, said Wednesday his Brazilian Social Democracy Party is studying the possibility of impeaching Ms. Rousseff, but hasn't come to a conclusion yet.

Congress would need evidence of direct involvement by Ms. Rousseff in the scheme before deciding to impeach, according to Rafael Cortez, a political scientist at São Paulo-based Tendencias Consultant Group.

"Just because party members were involved in irregularities doesn't mean Dilma was," he said. "But [Vaccari's arrest] obviously weakens her government even more, though given her low approval ratings, she can't fall much farther."

Ms. Rousseff's government is trying to pass a series of unpopular economic measures intended to cut its budget deficit and spur a recovery. She faces resistance from members of the PT and allied parties in Congress, many of whose members are under investigation in the scandal, and her political weakness is making that effort more difficult, Mr. Cortez said.

Police said at a news conference that they arrested Mr. Vaccari at his São Paulo home early Wednesday morning and brought his wife in for questioning, and also arrested three other people.

Police last year arrested several executives of the construction companies alleged to have overcharged Petrobras, as well as executives of the oil company, some of whom have turned state's evidence. Petrobras has said it considers itself a victim of the alleged scheme and is cooperating with authorities.

Five different witnesses have testified that Mr. Vaccari was involved in illegal activities for the PT, inspector Igor Romario de Paula of the Federal Police said at the news conference.

Paulo Trevisani and Luciana Magalhaes contributed to this article.

Write to Rogerio Jelmayer at rogerio.jelmayer@wsj.com and Jeffrey T. Lewis at jeffrey.lewis@wsj.com

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