PreOmics Introduces New ENRICH-iST Kits and Novel Workflow for Unbiased, Affordable Plasma and Serum Proteomics by LC-MS
05 Junho 2023 - 8:00AM
Business Wire
- ENRICH-iST enables unbiased enrichment of lower abundant plasma
and serum proteins by 50% to more than 100% in human and rodent
plasma proteomics in a cost-effective, fast 5-hour workflow
- ENRICH-iST utilizes non-functionalized paramagnetic microbeads
supporting automated platforms
- ENRICH-iST enables protein enrichment and tryptic digestion on
only 20 microL of starting material
At ASMS 2023, PreOmics GmbH announces the launch of its new
ENRICH-iST™ kits, which enable biologically unbiased enrichment of
lower abundant plasma and serum proteins onto non-functionalized
paramagnetic particles, enhancing typical depths of plasma
proteomics analysis by 50% to more than 100%, compared to neat
plasma or serum proteomics without depletion or enrichment. The
novel ENRICH-iST workflow enables fast and robust processing of up
to 96 plasma samples in parallel for reproducible large-cohort
translational and clinical research and validation. The affordable
ENRICH-iST routine enrichment workflow nicely complements existing
ultra-deep plasma proteomics discovery research workflows that
either use automated bead depletion or nanoparticle array
enrichment.
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ENRICH-iST Kit (displayed in
environmentally friendly packaging) is available as an 8x version
or as a 96x version for high-throughput plasma proteomics. For both
human plasma and serum samples, a significant increase in peptide
and protein identification was observed, comparing neat plasma and
serum with enriched samples. (Graphic: Business Wire)
The ENRICH-iST technology offers a simple, fast (<5h total)
and cost-effective solution for proteomic sample processing of
plasma or serum from various species, including human, rodents,
NHPs, dogs and pigs. Requiring only 20 microL of starting material,
it consistently delivers reproducible, enhanced results for LC-MS
analysis, e.g., by DIA-PASEF timsTOF 4D Proteomics mass
spectrometry. Utilizing non-functionalized paramagnetic microbeads,
the technology enables biologically unbiased enrichment of lower
abundant proteins, and supports liquid handling automation
platforms, such as Tecan FLUENT systems.
Dr. Andreas-David Brunner, Drug Discovery Sciences,
Boehringer Ingelheim Pharma GmbH & Co. KG, states: “We
routinely work with biofluid cohorts collected from preclinical
species. The novel ENRICH technology from PreOmics allowed us to
achieve reliable and in-depth proteomic data without laborious
method development. The new kit worked out of the box; the workflow
is intuitive and can be completely automated. This solution allows
us to standardize our processes completely, and significantly
improves our throughput.”
In a benchmark study, plasma and serum samples from human, mouse
and rat specimens (from suppliers Sigma Aldrich, BioIVT and
Diaserve Laboratories GmbH) were processed using the novel
ENRICH-iST workflow in comparison to antibody-based depletion, as
well as to unprocessed neat samples. Peptides were analyzed on a
timsTOF HT mass spectrometer (Bruker Daltonics) in DIA-PASEF mode
using a 30-minute nanoLC gradient. The ENRICH-iST workflow was
shown to increase the proteomic depth of peptides and proteins 1.5x
– 2.2x -fold in comparison to neat samples. Typical Coefficients of
Variation (CV) within replicates were below 12%, emphasizing high
repeatability of the ENRICH-iST workflow. Matching the identified
proteins with the plasma proteome database revealed a superior
coverage for the ENRICH-iST workflow over neat and depleted samples
with identified proteins covering a larger dynamic range.
ENRICH-iST showed higher precision over the measured abundance
range in comparison to the depletion workflow.
Dr. Catherine Nury, Manager Proteomics, Philip Morris
International, Switzerland, comments: “We tested several
options for biomarker discovery on plasma samples, but many were
long and tedious. With the novel ENRICH technology, we have a fast,
efficient, and reliable enrichment workflow to get deeper into the
proteome from human plasma and serum. We use the ENRICH-iST 96x
kits and are delighted by the reproducibility and ease of the kit.
We really enjoyed working with the PreOmics team, who are always
there to support us. We would strongly recommend the kit to other
plasma and serum proteomics users.”
Automation is key for high-throughput plasma proteomics and
Luca Valeggia, Senior Vice President - Lab Automation, at
Tecan, adds: “We are delighted to continue our collaboration
with PreOmics. By automating their new ERICH-iST kit on our Fluent
workstation, we’re enabling robust and reproducible high-throughput
sample preparation for large cohort studies, helping to further
accelerate progress in this rapidly advancing field.”
Have a look yourself: PreOmics representatives will be at ASMS
booth #827 to showcase the ENRICH-iST application note.
Additional information can be accessed at
www.preomics.com/products/enrich-ist.
About PreOmics GmbH
PreOmics empowers our clients in life sciences to establish
biological knowledge through efficient, reliable solutions and
workflows that set the standard for protein analysis. Our team
spirit, energy, and commitment empower us to be both creative and
quality focused – a trusted partner with deeply rooted scientific
experience. We envisage a future with revolutionary proteomic
discovery processes open for everyone. Tools that reveal hidden
causes of diseases, ensure sustainable nutrition, and provide
diagnoses that enhance lives and society.
Website: www.preomics.com Address: Am Klopferspitz 19 | 82152
Planegg/Martinsried | Germany
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version on businesswire.com: https://www.businesswire.com/news/home/20230605005320/en/
Dr. Garwin Pichler, Founder & Managing Director PreOmics
GmbH Email: info@preomics.com
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